How to use flat field correction

Flat field correction is used to correct the inhomogeneous background caused by the microscope or illuminations. Please remove the sample first and capture the inhomogeneous background images. Then put the samples on the microscope and enable the flat field correction. Please be sure that the sample is removed when the background images are taken.

When condition is changed, including optical system, illumination, exposure time or gain, FFC must be performed again in new condition.

1. Remove the sample first to get the inhomogeneous background.

2. Set the numbers of the background images and these inhomogeneous background images are going to be averaged and used as reference as FFC.

3. Click “Enable” button when “Enable” button is gray.

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4. Put the sample back.

Please check the results as below for your reference.

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